Journal: Cell research

Article Title: Pancreas-specific Pten deficiency causes partial resistance to diabetes and elevated hepatic AKT signaling.

doi: 10.1038/cr.2009.42

Figure Lengend Snippet: Figure 4 Liver proteomics analysis of PPKO and control mice. (A) Silver-staining images of hepatic proteins. Proteins from liver homogenates of 1-month-old mice (n = 6) were mixed together for 2-DE PAGE analysis. The hepatic proteins were sep- arated by isoelectric focusing (pH 3 to 10, horizontal axis) and 12.5% SDS-PAGE (vertical axis). The differential protein spots were showed in the image of PPKO mice. (B) Identities of the six differentially expressed proteins and fold changes revealed by proteomics. P ≤ 0.05. (C) Four differentially expressed proteins were confirmed by western blot of protein lysates from the liver of 1-month-old PPKO and control mice (n = 3). Blots were probed with antibodies that recognized NFYC, GRP78, CPS, FIS1, and ACTIN. *P < 0.05.

Article Snippet: Antibody to CPS was from Santa Cruz Biotechnology, to FIS1 was from MBL, to GRP78 was from Calbiochem, and to NFYC was from Protein Tech Group, Inc.

Techniques: Control, Silver Staining, SDS Page, Western Blot

Journal: Molecular Neurobiology

Article Title: Mbnl1 Protects Against Cerebral Ischemia–Reperfusion Injury by Modulating Microglia/Macrophage Polarization via NF-κB Pathway

doi: 10.1007/s12035-025-05180-1

Figure Lengend Snippet: Mbnl1 can reduce infarct area and improve cognitive function in mice. A TTC staining was used to detect the changes in infarct area of brain tissue in the model group and other groups of mice. The upper figure shows different sections in the brain tissue of a representative mouse in each group ( n = 6). B HE staining was used to observe the damage in the brain tissue of mice in each group ( n = 6). C Laser speckle blood flow imaging technology (LSCI) was used to detect the changes in cerebral blood flow (CBF) in the brain tissue of mice in each group ( n = 6). D The fatigue test was used to detect the duration of the balance and motor coordination ability of each group of mice on the rotating rod, that is, the time required for the animals to fall off the rotating rod ( n = 10). E The T-maze pull test was used to evaluate the use and force distribution of the forelimbs when mice pulled on the T-bar ( n = 10). F Neurological function of mice was scored at 24 h, 1 day, 3 days, and 7 days after infarction ( n = 10). G At 3 days and 7 days after infarction, the cognitive function of mice was evaluated through the Y-maze experiment ( n = 10) (compared between the two groups, ns represents no statistical significance,* P < 0.05, **** P < 0.0001)

Article Snippet: The mice were then positioned in a stereotactic frame and scanned using a laser speckle imaging system (Pericam PSIHR, PSIH-10129, PeriMed AB, Sweden).

Techniques: Staining, Imaging